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1 article(s) from Ding, Yun

Single pyrimidine discrimination during voltage-driven translocation of osmylated oligodeoxynucleotides via the α-hemolysin nanopore

Yun Ding and
Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 91–101, doi:10.3762/bjnano.7.11

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Scheme 1: OsBp with dTMP. Reaction of osmium tetroxide with 2,2’-bipyridine forms a reactive complex (OsBp or...

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Figure 1: Strategy for sequencing DNA using the osmylated strands. All sequences shown refer to deoxybases; f...

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Figure 2: Reaction of 12.6 mM OsBp (the highest concentration of OsBp used under our conditions) with 20 μM dA...

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Figure 3: Capillary electrophoresis (CE) overlapping traces of oligodeoxynucleotides pGEX3’-dA₂₅ intact and p...

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Figure 4: Translocation time histograms for four different oligos: Three are oligodeoxyadenylates, dA₁₀XdA₉ w...

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Figure 5: Representation of osmylated DNA strands to illustrate the practically parallel line-up of the OsBp ...

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Figure 6: Heat plots, normalized residual current, I_r/I₀, vs translocation time, t (ms), for the deoxyoligos ...

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Figure 7: Sample I–t traces for the control dA₂₀ and for dA₂₅-pGEX3’ R1 (with 4T(OsBp)) to show (a) continuou...

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Published 22 Jan 2016

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